| 金瑾1，秦令祥2，隋志方1，刘延奇3.响应面法优化高压脉冲电场协同双酶法提取乳木果油工艺及其品质分析[J].中国油脂,2025,50(7):.[JIN Jin1，QIN Lingxiang2，SUI Zhifang1，LIU Yanqi3.Optimization of high voltage pulsed electric field combined dual enzymes method extraction of shea butter by response surface methodology and its quality analysis[J].China Oils and Fats,2025,50(7):.] |
| 响应面法优化高压脉冲电场协同双酶法提取乳木果油工艺及其品质分析 |
| Optimization of high voltage pulsed electric field combined dual enzymes method extraction of shea butter by response surface methodology and its quality analysis |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240230 |
| 中文关键词: 乳木果油 高压脉冲电场 双酶 脂肪酸 生物活性成分 |
| 英文关键词:shea butter high voltage pulsed electric field dual enzymes fatty acid bioactive component |
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| 中文摘要: |
| 旨在为乳木果油的高效提取提供参考，以乳木果为原料，采用高压脉冲电场协同双酶法提取乳木果油，通过单因素试验研究酶种类、电场强度、双酶添加量、酶解温度对乳木果油得率的影响，并采用响应面法优化提取工艺。同时对最佳工艺下提取的乳木果油的理化指标、脂肪酸组成和生物活性成分含量进行分析。结果表明：高压脉冲电场协同双酶法提取乳木果油最佳工艺为电场强度20 kV/cm、双酶（纤维素酶与果胶酶质量比1∶ 1）添加量2.0%、酶解温度50 ℃，在此条件下乳木果油得率为48.69%；所得乳木果油酸值、过氧化值均符合食用植物油国标要求，乳木果油主要脂肪酸为硬脂酸、油酸、亚油酸、棕榈酸和花生酸，不饱和脂肪酸含量为47.85%；乳木果油含有4.39%的萜烯醇、2.97%的β-香树脂素、1.52%的羽扇豆醇、1.48%的羊毛甾醇等生物活性成分。综上，高压脉冲电场协同双酶法提取乳木果油可行，产品品质较好。 |
| 英文摘要: |
| Aiming to provide a reference for the efficient extraction of shea butter, shea fruit was used as raw material, and high voltage pulsed electric field combined dual enzymes method was used to extract shea butter. The effects of enzyme type, electric field intensity, dual enzymes dosage, and enzymatic hydrolysis temperature on the yield of shea butter were investigated by single factor experiment. The extraction process was optimized by response surface methodology. In addition, the physicochemical indicators, fatty acid composition and bioactive component content of the shea butter prepared under the optimal conditions were analyzed. The results showed that the optimal process for extracting shea butter were obtained as follows: electric field intensity 20 kV/cm, dual enzymes (a mass ratio of cellulase to pectinase 1∶ 1) dosage 2.0%, and enzymatic hydrolysis temperature 50 ℃. Under these conditions, the yield of shea butter was 48.69%, and the acid value and peroxide value of the shea butter met the requirements of the national standard for edible vegetable oil.The main fatty acids of the shea butter included stearic acid, oleic acid, linoleic acid, palmitic acid, and arachidic acid, with unsaturated fatty acid content at 47.85%. The shea butter also contained 4.39% terpene alcohol, 2.97% β-amyrin, 1.52% lupeol, and 1.48% lanosterol, and other bioactive components. In summary, the method of extracting shea butter using high voltage pulsed electric field combined dual enzymes is feasible, and the product quality is good. |
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