| 吾惠婷，许先敏，王钰翎，贾文敬，郑永标，黄建忠，舒正玉.蛋黄胆固醇的分离及结构鉴定[J].中国油脂,2025,50(7):.[WU Huiting, XU Xianmin, WANG Yuling, JIA Wenjing,
ZHENG Yongbiao, HUANG Jianzhong, SHU Zhengyu.Isolation and structural identification of cholesterol from egg yolk[J].China Oils and Fats,2025,50(7):.] |
| 蛋黄胆固醇的分离及结构鉴定 |
| Isolation and structural identification of cholesterol from egg yolk |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230567 |
| 中文关键词: 蛋黄油 胆固醇 分离 核磁共振 结构鉴定 |
| 英文关键词:egg yolk oil cholesterol isolation nuclear magnetic resonance structural identification |
| 基金项目:国家自然科学基金项目（31370802）；福建省自然科学基金项目（2021J01169）；福州市农业科技项目（2021-N-210） |
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| Author Name | Affiliation | | WU Huiting, XU Xianmin, WANG Yuling, JIA Wenjing,
ZHENG Yongbiao, HUANG Jianzhong, SHU Zhengyu | National and Local Joint Engineering Research Center for Industrial Microbial Fermentation Technology,
College of Life Science, Fujian Normal University, Fuzhou 350117, China |
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| 中文摘要: |
| 为探索快速、操作简单的鸡蛋黄中胆固醇的提取分离工艺，采用二元有机溶剂乙醇-正己烷混合溶剂萃取蛋黄中的油脂，以胆固醇含量为指标，通过单因素实验对蛋黄油萃取工艺条件进行优化，利用硅胶色谱柱对蛋黄油中胆固醇进行分离纯化，并通过核磁共振（NMR）进行结构鉴定。结果表明：蛋黄油最佳萃取工艺条件为乙醇与正己烷体积比1∶ 1、液料比3∶ 1，在此条件下胆固醇含量为21.14 mg/g，萃取率达100%；蛋黄油经硅胶色谱柱梯度洗脱后可获得单一组分，经核磁共振氢谱(1H NMR)和碳谱(13C NMR)初步鉴定其分子结构为27个碳原子组成的具有甾体母核的固醇。综上，二元有机溶剂萃取结合硅胶色谱柱分离可快速制备蛋黄胆固醇。 |
| 英文摘要: |
| In order to explore a fast and simple process for the extraction and separation of cholesterol from egg yolk, the oil in egg yolk was extracted with a binary organic solvent (ethanol-n-hexane mixed solvent), and the cholesterol content was used as an index, the oil extraction conditions were optimized by single factor experiment. The cholesterol in the egg yolk oil was isolated and purified using silica gel column chromatography, and its structural identification was carried out by nuclear magnetic resonance (NMR). The results showed that the optimal extraction conditions for egg yolk oil were volume ratio of ethanol to n-hexane 1∶ 1 and liquid to material ratio 3∶ 1. Under these conditions, the cholesterol content was 21.14 mg/g, and the extraction rate was 100%. A single component was obtained from the egg yolk oil after gradient elution on a silica gel column,and its molecular structure was initially identified as a steroidal parent core sterol composed of 27 carbon atoms by nuclear magnetic resonance hydrogen spectroscopy (1H NMR) and carbon spectroscopy (13C NMR). In conclusion, the binary organic solvent extraction combined with silica gel column chromatography can rapidly prepare cholesterol from egg yolk. |
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