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Effect of high hydrostatic pressure on ethanol-denatured soybean 11S globulin
  
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KeyWord:soybean 11S globulin  ethanol-denaturation  high hydrostatic pressure  soluble aggregation  unfolding  
FundProject:广东省重大专项
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Abstract:
      Effects of high hydrostatic pressure(HHP) on the solubility and structure of ethanol-denatured soybean 11S globulin were investigated.Soybean 11S globulin was denatured by 65% ethanol with ratio of 1:15.The solubility of ethanol-denatured soybean 11S globulin was 14.24%,and 300 MPa HHP treatment improved significantly its solubility to 95.5%.Gel permeation chromatograph showed that soluble-aggregations of various relative molecular weights were formed at different pressure.The analysis of fluorescence and c...
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