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| 脂肪酶催化大豆卵磷脂和亚油酸酯交换反应的研究 |
| Lipase catalyzed transesterification of soybean phosphatidylcholine and linoleic acid |
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| DOI： |
| 中文关键词: 卵磷脂 亚油酸 酶改性 酯交换 |
| 英文关键词:phosphatidylcholine linoleic acid enzymatic modification transesterification |
| 基金项目:江苏省科技型中小企业创新资金项目 |
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| 中文摘要: |
| 采用Lipozyme TL IM酶催化大豆卵磷脂(90%)和亚油酸(95%)进行酯交换反应,以制备富含亚油酸的卵磷脂.探讨了大豆卵磷脂质量浓度(甲苯为溶剂)、底物摩尔比(n(亚油酸):n(大豆卵磷脂))、酶添加量(以底物总质量为基准)、水分添加量(以酶质量为基准)、反应时间、反应温度对酯交换反应的影响.优化后的反应条件为:大豆卵磷脂质量浓度0.25g/ml,底物摩尔比6:1,酶添加量20%,水分添加量3%,反应时间60h,反应温度65℃.在最优反应条件下最终获得82.85%亚油酸含量的卵磷脂产品. |
| 英文摘要: |
| Immobilized lipase Lipozyme TL IM-catalyzed transesterification of soybean phosphatidylcholine(PC,90%) with linoleic acid(LA,95%) was carried out in toluene.The six major factors of phosphatidylcholine concentration(toluene as a solvent),substrate ratio(molar ratio of LA to PC),lipase dosage(based on the mass of substrates),water addition(based on the mass of lipase),reaction time and reaction temperature were discussed.The optimum reaction conditions were as follows: PC concentration 0.25 g/mL,molar ratio ... |
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