High acid rice bran oil was catalyzed to prepare rice bran oil rich in diacylglycerol(DAG)by lipase in a solvent-free system.The effects of lipase,esterification agent,mass ratio of substrate,reaction temperature,reaction time and lipase dosage on the content of DAG and the residual content of free fatty acid(FFA) were investigated.The reaction conditions were optimized by single factors experiment and response surface methodology as follows:immobilized Lipozyme RM IM used as a catalyst,glyceryl monooleate ... |