A qualitative and quantitative determination of the 1,3-diacylglycerol(1,3-DG) in milk fat was established.The 1,3-DG was effectively separated and qualitatively identified by TLC,and then was quantitatively analyzed by GPO-PAP.The RSD of the method was no more than 3.78% and the recovery was 86.73%-101.83%.The method was accurate,precise,rapid and suitable for the analysis of 1,3-DG in milk fat. |