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Determination of palm oil adulterated in peanut oil with the TaqMan probe -based RT- PCR method
  
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KeyWord:RT-PCR  TaqMan probe  MT3-B gene  palm oil  peanut oil  
FundProject:广东省科技计划资助项目，国家质检公益性行业科研专项项目，广东省质量技术监督局科技资助项目，湖南省科技项目，湖南省科技计划项目
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Abstract:
      The method of Real-time fluorescence quantitative polymerase chain reaction(RT-PCR) with TaqMan fluorescent probe was chosen to fast detect the amount of palm oil mixed in peanut oil.MT3-B gene of palm was selected as target gene to detect palm oil from peanut oil.The primers of MT3-B and TaqMan probe were designed,MT3-B gene reconstructed plasmid was built as absolute quantitative criteria for quantitative RT-PCR to establish standard curve.Peanut oil blended with 1%-40% concentration gradient palm oil was...
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