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| TaqMan探针荧光定量PCR检测花生油中掺入棕榈油的研究 |
| Determination of palm oil adulterated in peanut oil with the TaqMan probe -based RT- PCR method |
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| DOI： |
| 中文关键词: 荧光定量PCR TaqMan探针 MT3 -B基因 棕榈油 花生油 |
| 英文关键词:RT-PCR TaqMan probe MT3-B gene palm oil peanut oil |
| 基金项目:广东省科技计划资助项目，国家质检公益性行业科研专项项目，广东省质量技术监督局科技资助项目，湖南省科技项目，湖南省科技计划项目 |
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| 中文摘要: |
| 根据棕榈内源基因MT3 -B设计引物和TaqMan探针,采用基因重组技术构建用于检测棕榈基因MT3 -B的重组质粒作为绝对定量标准品,建立标准曲线,对花生油中掺入棕榈油1% ～40%梯度混合油品提取DNA进行棕榈成分定量检测.结果表明,重组质粒标准品荧光定量标准曲线对数线性回归分析相关系数(R2)为0.996;花生油中掺入棕榈油达到5%时,可检出每亳升混合油品中棕榈MT3 -B基因17.431 copies,检测的重复性和特异性好. |
| 英文摘要: |
| The method of Real-time fluorescence quantitative polymerase chain reaction(RT-PCR) with TaqMan fluorescent probe was chosen to fast detect the amount of palm oil mixed in peanut oil.MT3-B gene of palm was selected as target gene to detect palm oil from peanut oil.The primers of MT3-B and TaqMan probe were designed,MT3-B gene reconstructed plasmid was built as absolute quantitative criteria for quantitative RT-PCR to establish standard curve.Peanut oil blended with 1%-40% concentration gradient palm oil was... |
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