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Ultrasonic-assisted extraction of tea seed protein
  
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KeyWord:tea seed protein  alkaline extraction  extraction rate  ultrasonic-assist
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Author NameAffiliation
HU Wanhua  
ZHANG Shihong  
HE Dongping,etc  
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Abstract:
      The influencing factors including ultrasonic frequency, granularity of material, extraction temperature, extraction time, solid-liquid ratio and extraction pH were studied in the ultrasonic-assisted extraction process of tea seed protein. The results indicated that the most significant factor was extraction pH, followed by extraction temperature, extraction time, liquid-solid ratio and ultrasonic frequency, and the optimal extraction conditions were as follows: ultrasonic frequency 35 kHz, granularity of material 60 mesh, extraction pH 9.5, solid-liquid ratio 1∶14, extraction temperature 45℃, extraction time 1.5 h. Under the optimal conditions, the extraction rate of tea seed protein could reach 89.01% and the content of protein was 76%.
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