| Conventional liposomes of coix seed oil prepared by ethanol injection was coated by carboxymethyl chitosan (CMCT). The preparation of CMCT-coated liposomes containing coix seed oil was optimized by orthogonal experiment with coating efficiency as index on the basis of single factor experiment. The results indicated that the optimal preparation conditions were as follows: mass ratio of CMCT to phospholipid (PC) 5∶4, core material dosage 15%, phosphate buffer pH 6.5, emusifier dosage 0.04%(mass ratio of tween 80 to polyethylene glycol 400 1∶4). The CMCT-coated liposomes containing coix seed oil obtained under the optimal conditions were characterized with high embedding efficiency （86.78%）, heterogeneous size distribution （145.6 nm）, eligible Zeta potential （-30.4 mV） and excellent coating efficiency （99.18%）. In addition, transmission electron microscopy showed that these coated liposomes were spherical particles with the existence of CMCT layers surrounding on the surface of conventional liposomes. The CMCT layers could enhance the stability of conventional liposomes greatly. |
