EPA and DHA glycerides in fish oil from cobia viscera were enriched by lipase OF hydrolysis and the hydrolysis parameters were optimized using neural network. The results showed that when the mass ratio of water to oil was 3∶1, reaction temperature was 40-45℃ and dosage of lipase was 1.5% in the reaction system of buffer solution (pH 7.0), the total content of DHA and EPA in the enriched fish oil was 32.10%. The enriched fish oil had the characteristics of brilliant yellow color, clear and transparent with slight fishy smell, and the physical and chemical indexes were accordance with the secondary standard of refined fish oil in SC/T 3502—2000. |