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| 鱼汁豆粕混合物限定混菌固态发酵工艺的研究 |
| Limited mixed microbial solid-state fermentation of stickwater and soybean meal mixture |
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| DOI： |
| 中文关键词: 固态发酵；鱼汁；豆粕 饲料多肽；品质分析 |
| 英文关键词:solid-state fermentation stickwater soybean meal feed polypeptide quality analysis |
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| 中文摘要: |
| 研究限定枯草芽孢杆菌和乳酸杆菌固态发酵鱼汁和豆粕的混合物，转化饲料多肽。以可溶性多肽含量和胰蛋白酶抑制因子（TI）的降解率为检测指标，通过正交实验确定其最佳发酵条件为：发酵温度35℃，接种量8%，豆粕与鱼汁浓缩液（粗蛋白含量20%）质量体积比（g/mL）1∶1，发酵时间60 h。对最佳发酵条件下的产物品质进行检测，其中可溶性多肽含量达到23.54%，TI降解率为93.4%，益生菌数量达8.7×109 CFU/g。通过对比发酵原料和产物，结果表明鱼汁和豆粕混合物经混合菌发酵后，其品质得到明显改善。 |
| 英文摘要: |
| The process of preparing feed polypeptide from the mixture of stickwater and soybean meal fermented by limited mixed Bacillus subtilus and aobacillus plantarum L solid-state fermentation was studied. With soluble polypeptide content and trypsin inhibitor (TI) degradation rate as detection indicators, the optimal fermentation conditions were determined by orthogonal test as follows: fermentation temperature 35℃, inoculum size 8%, ratio of soybean meal mass to stickwater concentrated solution (crude protein content 20%) volume 1∶1, fermentation time 60 h. Under the optimal fermentation conditions, the content of soluble polypeptide was 23.54%, the TI degradation rate was 93.4%, and the number of probiotics reached 8.7×109 CFU/g. Comparing the raw materials with fermentation products, the results showed that the quality of stickwater and soybean meal mixture fermented by mixed bacteria was greatly improved. |
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