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Limited mixed microbial solid-state fermentation of stickwater and soybean meal mixture
  
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KeyWord:solid-state fermentation  stickwater  soybean meal  feed polypeptide  quality analysis
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Author NameAffiliation
YUAN Keping  
MAO Tao  
CAO Xixiu，etc  
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Abstract:
      The process of preparing feed polypeptide from the mixture of stickwater and soybean meal fermented by limited mixed Bacillus subtilus and aobacillus plantarum L solid-state fermentation was studied. With soluble polypeptide content and trypsin inhibitor (TI) degradation rate as detection indicators, the optimal fermentation conditions were determined by orthogonal test as follows: fermentation temperature 35℃, inoculum size 8%, ratio of soybean meal mass to stickwater concentrated solution (crude protein content 20%) volume 1∶1, fermentation time 60 h. Under the optimal fermentation conditions, the content of soluble polypeptide was 23.54%, the TI degradation rate was 93.4%, and the number of probiotics reached 8.7×109 CFU/g. Comparing the raw materials with fermentation products, the results showed that the quality of stickwater and soybean meal mixture fermented by mixed bacteria was greatly improved.
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