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| Preparation of rice bran peptide from high-temperature rice bran meal |
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| KeyWord:high-temperature rice bran meal rice bran peptide hydrolysis preparation |
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| Abstract: |
| Using high-temperature rice bran meal as raw material，rice bran protein was extracted by alkaline extraction and acid precipitation，then rice bran protein was hydrolyzed to prepare rice bran peptide by alkaline protease. On the basis of single factor experiment，the hydrolysis conditions were optimized by quadratic regression orthogonal experiment as follows：hydrolysis temperature 55℃，enzyme dosage 0.3%(based on the mass of high-temperature rice bran meal)，pH 9.6，hydrolysis time 3 h. Under the optimal conditions，the hydrolysis degree of rice bran protein was 10.3%，the crude protein content of rice bran peptide was 85.7%,protein dispersion index(PDI)was 76.4%,nitrogen dissolving index(NSI)was 64.1%，and relative molecular weight of rice bran peptide between 180 and 3 000 accounted for 78.88%. |
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