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Optimization of immobilized lipase catalyzed transesterification of cottonseed oil for biodiesel production using response surface methodology
  
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KeyWord:cottonseed oil  lipase  biodiesel  response surface methodology
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Author NameAffiliation
LI Yingxia  
ZHAO Fengjuan  
NIU Qiuhong，etc  
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Abstract:
      The process of immobilized lipase catalyzed transesterification of cottonseed oil for biodiesel production was optimized by response surface methodology. With fatty acid methyl ester conversion rate as index, the effects of reaction time, reaction temperature, immobilized lipase amount (on the basis of cottonseed oil mass), molar ratio of methanol to cottonseed oil and volume ratio of tert-butanol to cottonseed oil on the conversion rate were studied. The optimal reaction conditions were determined as follows: reaction time 19.128 h, reaction temperature 37.801℃, immobilized lipase amount 12.070%, molar ratio of methanol to cottonseed oil 4.949, volume ratio of tert-butanol to cottonseed oil 0.323. The verification test showed that the conversion rate reached 92.9%, which had a high degree of agreement with the response suface methodology predicted value (94.3%).
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