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Antioxidant effects of wheat germ protein isolate hydrolysates against H2O2-induced oxidative stress in PC12 cells
  
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GUO Xu  
ZHU Kexue  
GUO Xiaona  
ZHOU Huiming  
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Abstract:
      Wheat germ protein isolate hydrolysates (WGPIH) were prepared under different enzymolysis time and the antioxidant effects of different WGPIH against the H2O2-induced oxidative stress in PC12 cells were compared through MTT method combined with their antioxidant activities in vitro, and the changes of the PC12 cells surface microstructures were observed by SEM. The results showed that the hydrolysate WGPIH5 produced by hydrolyze for 240 min possessed the highest antioxidant activity, its IC50value for ferrous iron-chelating activity was（0.37±0.05）mg/mL；TEAC value （Trolox） reached（0.81±0.01）mmol/L with 5 mg/mL WGPIH5; the autooxidation of linoleic acid was significantly inhibited by WGPIH5；WGPIH5 was able to attenuate H2O2
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