| The effects of docosahexaenoic acid-enriched phospholipids （DHA-PL） on lipid metabolism in diet-induced obese C57BL/6J mice model were investigated. DHA-PL were extracted from Sthenoteuthis oualaniensis spawns by Folch method and purified by silica gel column chromatography. The mice were assigned to three groups randomly: high-fat model group, 1% soybean phospholipids group and 1% DHA-PL group. After seven weeks of feeding, the contents of triglycerides （TG）, total cholesterol （TC） and high density lipoprotein cholesterol （HDL-C） in serum and the contents of TC, TG and PL in liver of the mice were meassured, and the activities of hepatic fatty acid synthase（FAS）, malic enzyme（ME）, glucose-6-phosphate dehydrogenase（G6PDH）, carnitine palmitoyl transterase （CPT）, peroxisomal β-oxidation enzymes and phosphatidic acid phophyhydrolase （PAP） were determined. The results showed that DHA-PL inhibited the accumulations of body weight （P＜0.01）, perirenal adipose tissue（P＜0.01） and epididymal adipose tissue （P＜0.01） of mice significantly, reduced the contents of TC （P＜0.01） in serum and hepatic TG （P＜0.01）, inhibited the activities of FAS （P＜0.01）, ME （P＜0.05） and PAP （P＜0.05） in liver markedly, and enhanced the activities of CPT （P＜0.01） and peroxisomal β-oxidation enzymes （P＜0.05）. The effect of DHA-PL on improving the lipid metabolism was superior to that of soybean phospholipids. DHA-PL played a good role in weight loss, and it could promote decomposition of lipid through inhibiting synthesis of lipid in liver and regulate lipid metabolism of obese mice effectively. |
