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| 固定化脂肪酶催化合成植物甾醇油酸酯的研究 |
| Synthesis of phytosterol oleate catalyzed by immobilized Candida rugosa lipase |
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| DOI： |
| 中文关键词: 植物甾醇油酸酯 脂肪酶 固定化 |
| 英文关键词:phytosterol oleate lipase immobilization |
| 基金项目: |
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| 中文摘要: |
| 以无纺布为固定化载体，以Candida rugosa脂肪酶为催化剂，催化植物甾醇与油酸酯化反应合成植物甾醇油酸酯。研究了反应温度、反应时间、底物摩尔比（油酸与植物甾醇摩尔比）、正己烷用量和酶用量对植物甾醇酯化率的影响。在单因素实验的基础上，经响应面实验优化反应条件。结果表明，植物甾醇油酸酯最佳合成条件为：反应温度50℃，反应时间18 h，底物摩尔比2∶1，正己烷用量1 mL，酶用量62.06 U/mg。在最佳条件下，植物甾醇酯化率可达98.36%。 |
| 英文摘要: |
| With non-woven fabric as immobilized carrier，esterification of phytosterols and oleic acid was catalyzed by Candida rugosa lipase. The effects of reaction temperature，reaction time，molar ratio of substrate （molar ratio of oleic acid to phytosterols），n-hexane dosage and enzyme dosage on esterification rate of phytosterols were studied. Based on single factor experiment，the optimal synthesis conditions of phytosterol oleate were obtained by response surface methodology as follows：reaction temperature 50℃，reaction time 18 h，molar ratio of substrate 2∶1，n-hexane dosage 1 mL，enzyme dosage 62.06 U/mg. Under the optimal conditions，the esterification rate of phytosterols could reach 98.36%. |
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