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Determination of diacylglycerol content in soybean oil by RP-HPLC-ELSD
  
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KeyWord:diacylglycerol  RP-HPLC  ELSD
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Author NameAffiliation
GAO Xiaohong  
ZHENG Yi  
YUAN Jian  
HE Rong  
WANG Lifeng  
JU Xingrong  
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Abstract:
      With crude soybean oil,degummed oil, bleached oil, deodorizated oil and first grade oil as raw materials, the dilinoelin (LL), 1-palmitin-3-linolein(1-P-3-L), diolein (OO) and 1-palmitin-3-olein (1-P-3-O) in these oils were determined by RP-HPLC-ELSD. The results showed that the linear correlation coefficients of the peak areas and the contents of the four diacylglycerols were 0.999 8, 0.998 7, 0.999 5, 0.996 0, respectively. The relative standard deviations and the recoveries of standard addition were 0.20%-2.00% and 102.2%-104.5%， respectively.
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