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High efficient extraction of DNA from plant oil
  
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KeyWord:plant oil  DNA extraction  transgene detection
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Author NameAffiliation
CAI Cuixia  
XIAO Weiwei  
WU Huihui  
ZHOU Linhua  
WU Yongbin  
GENG Qibin  
MA Wenli  
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Abstract:
      According to the features of low content,short sequence fragment and serious destruction of DNA in plant oil, a stable and high efficient extraction method of DNA from edible plant oil was established. DNA was extracted from plant oils using silicon film adsorption column method, and the endogenous genes in the DNA were amplificated by PCR to identify DNA quality, then the exogenous gene promoter CaMV35S was detected by PCR, LAMP and real-time fluorescent PCR to evaluate the method. The results showed that the DNA extracted by the method had reliable quality and it was successful to detect the promoter CaMV35S by PCR，LAMP and real-time fluorescent PCR. The DNA extracted from plant oil by the method could be used as the amplification template of PCR, LAMP and real-time fluorescent PCR for genetically modified ingredients detection. The method had items of stableness, effectiveness and safety which could provide the foundation for plant oil gene detection.
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