Preparation of cottonseed peptide and its free radical scavenging capacity
  
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KeyWord:cottonseed peptide  degree of hydrolysis  protease  free radical scavenging capacity
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LIANG Yongfei  
YU Ling  
YU Qing,etc  
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Abstract:
      The cottonseed protein prepared by alkali extraction and acid precipitation was hydrolyzed respectively by Alcalase, Neutrase, Pepsin and Papain, and the degrees of hydrolysis of cottonseed protein and scavenging capacities on free radicals of cottonseed peptide obtained were compared. The results showed that the cottonseed peptide produced by Neutrase presented stronger free radical scavenging activity. On the basis of single factor experiment,the preparation conditions of cottonseed peptide by Neutrase hydrolysis were optimized by orthogonal experiment as follows: hydrolysis time 5 h, hydrolysis temperature 45℃, pH 7.5, enzyme dosage 8 000 U(100 mL 5% of cottonseed protein solution). Under these conditions, the degree of hydrolysis of cottonseed protein reached 11.23%, the scavenging rates of cottonseed peptide on superoxide anion free radical and hydroxyl free radical were 72.48% and 89.01% respectively, and the scavenging rate of ten times-diluted cottonseed peptide solution on DPPH free radical was 84.67%. The free radical scavenging capacity of cottonseed peptide was similar to that of 0.5 mg/mL vitamin C.
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