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| 交联剂固定化假丝酵母脂肪酶及其催化反应研究 |
| Immobilization of Candida lipase by crosslinking agent and its catalytic reaction |
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| DOI： |
| 中文关键词: 脂肪酶 固定化 交联剂 催化 |
| 英文关键词:lipase immobilization crosslinking agent catalysis |
| 基金项目: |
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| 中文摘要: |
| 以价格低廉的无纺布为固定化载体，添加聚丙烯酸酯作为交联剂，生产固定化脂肪酶。这种固定化方法对脂肪酶的纯度等性质要求不高，制备工艺操作简单。交联剂对酶液的活力没有影响，且具有一定的成膜特性，能够为脂肪酶分子催化提供良好的微环境。固定化脂肪酶酶活损失较少，在25%以内。制备的固定化脂肪酶在棕榈酸异辛酯酯化反应体系可以重复催化达到47批次，酯化率在80%以上；在酯交换生产脂肪酸甲酯反应体系可以连续反应25批次，转酯化率在90%以上。 |
| 英文摘要: |
| Immobilized lipase was produced with non-woven fabrics as carrier and polyacrylic ester as crosslinking agent. The preparation process of the immobilization method was simple and the requirement for lipase properties was not high. Crosslinking agent had no effect on the lipase activity, and had certain film-forming property, which could provide good microenvironment for lipase catalysis and the loss of immobilized lipase activity was within 25%. The prepared immobilized lipase could be used repeatedly for fouty-seven batches in palmitic acid isooctyl ester reaction system, and the esterification rate was over 80%, while in the fatty acid methyl ester reaction system, the immobilized lipase could be used repeatedly for twenty-five batches with the esterification rate being over 90%. |
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