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| Optimization of aqueous enzymatic extraction of heepseed oil using Alcalase2.4L by response surface methodology |
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| KeyWord:hempseed aqueous enzymatic extraction Alcalase2.4L response surface methodology |
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| Abstract: |
| The aqueous enzymatic extractions of heepseed oils from Yunma No.1 and Bama Huoma were researched with Alcalase2.4L，Protamex，Neutrase0.8L，Celluclast1.5L and Viscozyme respectively or compositively. According to the oil extraction rate, Alcalase2.4L was selected as the best enzyme to hydrolyze Yunma No.1.Then，the effects of crushing times，ratio of water to material，enzyme dosage，initial enzymolysis pH，enzymolysis temperature and enzymolysis time on the oil extraction rate of hempseed were studied.The optimal enzymolysis conditions were obtained by response surface methodology as follows：crushing times 4（5 s for each times），ratio of water to material 3.5∶1，enzyme dosage 2.0%（based on the mass of heepseed），initial enzymolysis pH 7，enzymolysis temperature 70℃ and enzymolysis time 3.5 h. Under these conditions，the oil extraction rate of heepseed was 83.81%. |
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