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Extraction of rapeseed oil assisted with enzymatic pretreatment
  
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KeyWord:enzymatic pretreatment  rapeseed oil  mechanism
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ZHOU Hongxiang  
WU Fengzhi  
LIU Lin，dtc  
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Abstract:
      Four kinds of enzymes, Flavourzyme,Neutral Protease, Cellulase and Pectinase were screened out and recomposed according to the main components of rapeseed, and the optimal dosage ratio of Flavourzyme,Neutral Protease, Cellulase and Pectinase was obtained as 1.5%∶2.0%∶1.0%∶1.5% with oil extraction rate as index. Under the condition, the oil extraction rate of rapeseed was 91.73%. Then the factors, ratio of material to water, pH, enzymolysis time and enzymolysis temperature, were optimized as follows: ratio of material to water 1∶0.5, pH 6.0, enzymolysis temperature 50℃ and enzymolysis time 3.0 h. Under the optimal extraction conditions, the oil extraction rate was 95.62%. Finally the contents change of reducing sugar and amino nitrogen and the scanning electron microscopy prints in the process of enzymatic pretreatment were investigated to primarily validate the action mechanism of enzymes.
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