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Qualitative analysis of stigmasterol oxides in soybean oil by GC-MS
  
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KeyWord:stigmasterol  oxide  GC-MS
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SHI Fang  
JIN Qingzhe  
WANG Xingguo  
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Abstract:
      The qualitative analysis method of stigmasterol oxides in soybean oil was established. Soybean oil was oxidized under the oxidation conditions of stigmasterol in vegetable oil,and six kinds of stigmasterol oxides in the oxidized soybean oil were determined qualitatively by GC-MS with stigmasterol oxides prepared by stigmasterol standard as the standard control after the oxidized soybean oil was pretreated by cold saponification, organic solvents extraction, solid phase extraction and derivatization.The results showed the stigmasterol oxides could be separated well from soybean oil by the method with good reproducibility, therefore,the method could be applied to qualitative analysis of sterol oxides in vegetable oil.
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