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Improvement of biomass and oil content of Scenedesmus sp. by two-step cultivation
  
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KeyWord:Scenedesmus sp.  two-step cultivation  oil content  biomass
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Author NameAffiliation
LI Xiaomei  
LIAO Xinghui  
WANG Mingzi，etc  
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Abstract:
      Scenedesmus sp. was cultured to stationary phase under the conditions of low illumination intensity with nitrogen,and then cultured in medium with high illumination intensity and nitrogen deficiency to accumulate oil. The results showed that in nitrogenous BG11 medium with low illumination intensity,the optimal illumination intensity for the growth of Scenedesmus sp. was 2 000-2 400 lx,culturing for 10 d; in nitrogen deficiency BG11 medium with high illumination intensity, the suitable illumination intensity for oil accumulation of Scenedesmus sp. was 6 000-6 500 lx,culturing for 6 d. The biomass and oil content of Scenedesmus sp. cultured by two-step method were （0.957±0.126） g/L and （23.35±0.2）% respectively, increasing by 35.74% and 100% than that cultured by common one-step method.
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