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| Effect of combination of carbon and nitrogen on growth and lipid accumulation of P. tricornutum under mixotrophic cultivation |
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| KeyWord:P. tricornutum biomass lipid accumulation ammonium chloride glucose |
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| Abstract: |
| The effects of different combinations of carbon and nitrogen sources on the growth and lipid accumulation of P. tricornutum under mixotrophic cultivation were studied. The carbon source was glucose and the nitrogen source was ammonium chloride. The biomass of P. tricornutum was determined by dry weight method. The lipid was extracted by chloroform-methanol and the fatty acid composition of the lipid was analyzed by GC. The results showed that the cell yield coefficient based on consumption of ammonium chloride reduced with the mass concentration of glucose increasing, so the demand for nitrogen source dropped; under the conditions of mass concentration of glucose 0.5 g/L and concentration of ammonium chloride 1.0 mmol/L, the biomass and lipid content of P. tricornutum reached 1.25 g/L and 44.6% respectively; there was no significant effect of different combinations of carbon and nitrogen on the contents of polyunsaturated fatty acids and eicosapentaenoic acid of P. tricornutum lipid. |
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