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Recombinant expression of Clostridium perfringens phospholipase C and its application in degumming
  
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KeyWord:phospholipase C  Clostridium perfringens  recombinant expression  degumming  crude rapeseed oil
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Author NameAffiliation
RUI Lilian  
YU Zhenzhen  
LIANG Li，etc  
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Abstract:
      The phospholipase C gene from Clostridium perfringens was recombinationally expressed in E.coli BL21(DE3),and the expression conditions of recombinant Clostridium perfringens phospholipase C(Cp-PLC)were optimized.The results showed that under the conditions of absorbance of cells at 600 nm 1.0, lactose dosage 8 g/L, induction temperature 30℃ and induction time 32 h, the activity of recombinant Cp-PLC obtained reached 398.35 U/mL. The optimal degumming conditions of crude rapeseed oil with the recombinant Cp-PLC were obtained as follows: temperature 57℃, pH 5.0, enzyme dosage 600 U/kg, reaction time 1.5 h. Under these conditions, the phosphorus content of degummed rapeseed oil decreased to 5.66 mg/kg, which could fulfill the requirement of the following physical refining.
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