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产气荚膜梭菌磷脂酶C的重组表达及其脱胶应用
Recombinant expression of Clostridium perfringens phospholipase C and its application in degumming
  
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中文关键词:  磷脂酶C  产气荚膜梭菌  重组表达  脱胶  菜籽毛油
英文关键词:phospholipase C  Clostridium perfringens  recombinant expression  degumming  crude rapeseed oil
基金项目:
Author NameAffiliation
RUI Lilian  
YU Zhenzhen  
LIANG Li，etc  
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中文摘要:
      将产气荚膜梭菌(Clostridium perfringens)磷脂酶C(Cp-PLC)基因在大肠杆菌BL21(DE3)中进行了重组表达，并对重组Cp-PLC表达条件进行了优化。结果表明：当菌体生长至OD600为10时，添加8 g/L乳糖在30℃下诱导32 h，得到的重组Cp-PLC的酶活最大，为398.35 U/mL；重组Cp-PLC用于菜籽毛油脱胶的最佳条件为温度57℃、pH 5.0、加酶量600 U/kg、反应时间1.5 h；在最佳条件下，菜籽毛油的磷含量可降至5.66 mg/kg，能够满足物理精炼的要求。
英文摘要:
      The phospholipase C gene from Clostridium perfringens was recombinationally expressed in E.coli BL21(DE3),and the expression conditions of recombinant Clostridium perfringens phospholipase C(Cp-PLC)were optimized.The results showed that under the conditions of absorbance of cells at 600 nm 1.0, lactose dosage 8 g/L, induction temperature 30℃ and induction time 32 h, the activity of recombinant Cp-PLC obtained reached 398.35 U/mL. The optimal degumming conditions of crude rapeseed oil with the recombinant Cp-PLC were obtained as follows: temperature 57℃, pH 5.0, enzyme dosage 600 U/kg, reaction time 1.5 h. Under these conditions, the phosphorus content of degummed rapeseed oil decreased to 5.66 mg/kg, which could fulfill the requirement of the following physical refining.
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