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| Determination of aflatoxin B1 in edible vegetable oil by immunoaffinity column purification and HPLC-MS/MS |
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| KeyWord:immunoaffinity column purification HPLC-MS/MS aflatoxin B1 edible vegetable oil |
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| Abstract: |
| Immunoaffinity column purification and HPLC-MS/MS method was established to determine aflatoxin B1 in edible vegetable oil. Aflatoxin B1 was extracted from edible vegetable oil by 70% of methanol,then the extract was filtered and purified by immunoaffinity column before being injected into HPLC-MS/MS to determine aflatoxin B1. The results showed that calibration curve presented good linear relationship when the mass concentration of aflatoxin B1 ranged from 0.2 ng/mL to 10 ng/mL;the limit of detection（S/N=3） and the limit of quantification（S/N=10） of the method were 0.05 μg/kg and 0.2 μg/kg respectively,the average recovery of standard addition was in the range of 83.0%-94.8%, and the relative standard deviation was less than 7.8%. |
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