| The?Paeonia?suffruticosa?seed?oil?was?extracted?by?aqueous?enzymatic?method.?According?to?comparative?experiments，the?three-step?enzymolysis?combined?with?twice?demulsification?was?chose?as?the?optimal?process.?The?results?showed?that?the?extraction?conditions?of?Paeonia?suffruticosa?seed?oil?by?three-step?enzymolysis?combined?with?twice?demulsification?were?optimized?as?follows：5?g?Paeonia?suffruticosa?seed?powder,?ratio?of?material?to?water?1∶5,?dosage?of?cell?wall?polysaccharides?hydrolysis?enzyme?(ratio?of?Cellelase?to?Pectinase?2∶1)?1.5?mL，enzymolysis?time?2.5?h;?α-amylase?dosage?0.6?mL，enzymolysis?time?45?min;?alkaline?protease?dosage?0.18?g，enzymolysis?time?2?h;?using?freezing?and?thawing?demulsification?method，freezing?temperature?-20℃，freezing?time?18?h，thawing?temperature?50℃，thawing?time?2?h.?When?extracting?oil?from?200?g?of?Paeonia?suffruticosa?seed?powder?under?the?optimal?conditions，the?free?oil?yield?was?17.6%?and?the?total?oil?yield?was?25.4%.?The?Paeonia?suffruticosa?seed?oil?obtained?had?good?quality?in?terms?of?undetected?peroxide，the?acid?value?3.5?mgKOH/g，iodine?value?177.09?gI/100?g?and?saponification?value?173.07?mgKOH/g.?The?unsaturated?fatty?acids?content?of?Paeonia?suffruticosa?seed?oil?was?up?to?92.77%，in?which?the?contents?of?linolenic?acid，linoleic?acid?and?oleic?acid?were?37.33%,31.13%?and?24.31%?respectively.?The?aqueous?enzymatic?extraction?of?Paeonia?suffruticosa?seed?oil?had?the?items?of?the?seed?powder?without?drying?and?the?extraction?temperature?below?70℃?during?the?whole?process,which?could?reduce?the?chances?of?oil?oxidation. |
