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| 水酶法提取牡丹籽油的研究 |
| Aqueous enzymatic extraction of Paeonia suffruticosa seed oil |
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| DOI： |
| 中文关键词: 水酶法 牡丹籽油 GC-MS |
| 英文关键词:aqueous enzymatic method Paeonia suf fruticosa seed oil GC-MS |
| 基金项目: |
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| 中文摘要: |
| 采用水酶法从牡丹籽中提取牡丹籽油，通过对比实验，选择了三步酶解结合二次破乳的工艺流程。5?g牡丹籽粉三步酶解结合二次破乳提取牡丹籽油的优化条件为：料水比1∶5，细胞壁多糖水解酶（纤维素酶与果胶酶配比2∶1）加酶量1.5?mL，酶解时间2.5?h；α-中温淀粉酶加酶量0.6?mL，酶解时间45?min；碱性蛋白酶加酶量0.18?g，酶解时间2?h；冷冻解冻破乳法，-20℃冷冻18?h后50℃解冻2?h。在优化条件下取200?g牡丹籽粉提取牡丹籽油，其游离油得率达到17.6%，总油得率达到25.4%，所得牡丹籽油品质良好，未检出过氧化物，酸值（KOH）3.5?mg/g，碘值（I）177.09?g/100?g，皂化值（KOH）173.07?mg/g；牡丹籽油中不饱和脂肪酸含量达到92.77%，其中亚麻酸含量37.33%，亚油酸含量31.13%，油酸含量24.31%。水酶法提取牡丹籽油具有牡丹籽粉无需干燥，整个提油过程温度不超过70℃的优点，可大大减少提取过程中油脂的氧化。 |
| 英文摘要: |
| The?Paeonia?suffruticosa?seed?oil?was?extracted?by?aqueous?enzymatic?method.?According?to?comparative?experiments，the?three-step?enzymolysis?combined?with?twice?demulsification?was?chose?as?the?optimal?process.?The?results?showed?that?the?extraction?conditions?of?Paeonia?suffruticosa?seed?oil?by?three-step?enzymolysis?combined?with?twice?demulsification?were?optimized?as?follows：5?g?Paeonia?suffruticosa?seed?powder,?ratio?of?material?to?water?1∶5,?dosage?of?cell?wall?polysaccharides?hydrolysis?enzyme?(ratio?of?Cellelase?to?Pectinase?2∶1)?1.5?mL，enzymolysis?time?2.5?h;?α-amylase?dosage?0.6?mL，enzymolysis?time?45?min;?alkaline?protease?dosage?0.18?g，enzymolysis?time?2?h;?using?freezing?and?thawing?demulsification?method，freezing?temperature?-20℃，freezing?time?18?h，thawing?temperature?50℃，thawing?time?2?h.?When?extracting?oil?from?200?g?of?Paeonia?suffruticosa?seed?powder?under?the?optimal?conditions，the?free?oil?yield?was?17.6%?and?the?total?oil?yield?was?25.4%.?The?Paeonia?suffruticosa?seed?oil?obtained?had?good?quality?in?terms?of?undetected?peroxide，the?acid?value?3.5?mgKOH/g，iodine?value?177.09?gI/100?g?and?saponification?value?173.07?mgKOH/g.?The?unsaturated?fatty?acids?content?of?Paeonia?suffruticosa?seed?oil?was?up?to?92.77%，in?which?the?contents?of?linolenic?acid，linoleic?acid?and?oleic?acid?were?37.33%,31.13%?and?24.31%?respectively.?The?aqueous?enzymatic?extraction?of?Paeonia?suffruticosa?seed?oil?had?the?items?of?the?seed?powder?without?drying?and?the?extraction?temperature?below?70℃?during?the?whole?process,which?could?reduce?the?chances?of?oil?oxidation. |
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