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| 布那迪栅藻异养培养条件的优化和生化组成含量分析 |
| Optimization of heterotrophic cultivation conditions and biochemical components of Scenedesmus bernardii |
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| DOI： |
| 中文关键词: 布那迪栅藻；异养培养 总脂 碳水化合物；产率 |
| 英文关键词:Scenedesmus bernardii heterotrophic cultivation total lipid carbohydrates yield |
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| 中文摘要: |
| 研究了布那迪栅藻（Scenedesmus?bernardii）异养培养条件及不同碳源、氮源对其生化组成的影响。结果表明：异养培养时所需的最佳碳源为葡萄糖，其最适质量浓度为50.0?g/L；最佳氮源为酵母提取物，其最适质量浓度为2.7?g/L；摇瓶培养时所获得的最大生物量达24.8?g/L。布那迪栅藻异养培养时细胞内主要积累碳水化合物，其含量达到细胞干重的58.2%，最高产率为1.05?g/（L·d）；其次积累总脂，含量为细胞干重的30.9%，最高产率为0.64?g/（L·d）。 |
| 英文摘要: |
| The?heterotrophic?cultivation?conditions?of?Scenedesmus?bernardii?and?the?different?carbon?sources?and?nitrogen?sources?on?its?biochemical?components?were?studied.?The?results?showed?that?the?best?carbon?source?for?Scenedesmus?bernardii?under?heterotrophic?growth?was?glucose,?and?its?optimal?mass?concentration?was?50.0?g/L;?the?best?nitrogen?source?for?Scenedesmus?bernardii?under?heterotrophic?growth?was?yeast?extract,?and?its?optimal?mass?concentration?was?2.7?g/L.?Under?the?optimal?heterotrophic?growth?conditions,?the?maximum?biomass?was?up?to?24.8?g/L.The?carbohydrates?mainly?accumulated?in?the?cell?of?Scenedesmus?bernardii?under?heterotrophic?growth?conditions,?accounting?for?58.2%?of?the?cell?dry?weight,?and?the?highest?yield?was?1.05?g/(L·d);?the?next?was?the?total?lipid,?accounting?for?30.9%?of?the?cell?dry?weight,?and?the?highest?yield?was?0.64?g/(L·d). |
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