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大豆磷脂酰乙醇胺的分离及纯化
Separation and purification of soybean phosphatidylethanolamine
  
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中文关键词:  大豆粉末磷脂  磷脂酰乙醇胺  溶剂提取  冷冻纯化
英文关键词:soybean phospholipids powder  phosphatidylethanolamine  solvent extraction  freezing purification
基金项目:
Author NameAffiliation
WANG Qiang  
SHI Sujia  
HAN Haixia，etc  
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中文摘要:
      以提取卵磷脂（PC）后的大豆粉末磷脂为原料，以碱性乙醇为溶剂提取磷脂酰乙醇胺（PE），并进行冷冻纯化。在单因素实验的基础上，以PE得率为指标，响应面优化实验确定了PE的最佳提取条件为：提取温度45℃，料液比1∶20，乙醇碱浓度V（无水乙醇）∶V（25%氨水）=100∶2，提取时间50?min，提取次数2次。在最佳条件下，PE得率为79.53%，含量为48.42%；当冷冻温度为-15℃，冷冻时间为8?h时，PE含量提升到72.73%，得率为66.71%。
英文摘要:
      With?soybean?phospholipids?powder?as?raw?material,?in?which?phosphatidylcholine?was?extracted,?phosphatidylethanolamine（PE）?was?extracted?by?alkaline?ethanol?and?purified?by?freezing.?With?yield?of?PE?as?the?indicator,?based?on?the?single?factor?experiment,?the?optimal?extraction?conditions?of?PE?were?obtained?by?response?surface?methodology?as?follows:?extraction?temperature?45?℃,?solid-liquid?ratio?1∶20,concentration?of?alkaline?ethanol?2.00?mL?of?25%?ammonia?per?100?mL?ethanol,?extraction?time?50?min?and?extraction?times?twice.?Under?these?conditions,?the?yield?and?content?of?PE?were?79.53%?and?48.42%?respectively.?Meanwhile,?under?the?conditions?of?freezing?temperature?-15?℃?and?freezing?time?8?h,?the?content?of?PE?improved?to?72.73%?and?the?yield?was?66.71%.
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