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Separation and purification of soybean phosphatidylethanolamine
  
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KeyWord:soybean phospholipids powder  phosphatidylethanolamine  solvent extraction  freezing purification
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Author NameAffiliation
WANG Qiang  
SHI Sujia  
HAN Haixia，etc  
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Abstract:
      With?soybean?phospholipids?powder?as?raw?material,?in?which?phosphatidylcholine?was?extracted,?phosphatidylethanolamine（PE）?was?extracted?by?alkaline?ethanol?and?purified?by?freezing.?With?yield?of?PE?as?the?indicator,?based?on?the?single?factor?experiment,?the?optimal?extraction?conditions?of?PE?were?obtained?by?response?surface?methodology?as?follows:?extraction?temperature?45?℃,?solid-liquid?ratio?1∶20,concentration?of?alkaline?ethanol?2.00?mL?of?25%?ammonia?per?100?mL?ethanol,?extraction?time?50?min?and?extraction?times?twice.?Under?these?conditions,?the?yield?and?content?of?PE?were?79.53%?and?48.42%?respectively.?Meanwhile,?under?the?conditions?of?freezing?temperature?-15?℃?and?freezing?time?8?h,?the?content?of?PE?improved?to?72.73%?and?the?yield?was?66.71%.
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