|
| Removal of interfering components by C18 column in determination of 3-chloropropane-1,2-diol fatty acid esters |
| |
| DOI： |
| KeyWord:3-chloropropane-1,2-diol fatty acid esters C18 column interfering component HPLC |
| FundProject: |
|
| Hits: 1576 |
| Download times: 0 |
| Abstract: |
| The?effects?of?solid?phase?extraction?column?and?sillca?gel?column?chromatography?on?the?separation?of?oil?sample?were?studied?and?compared?so?as?to?remove?the?interfering?components?from?oil?by?preseparation?process?in?the?determination?of?3-chloropropane-1,2-diol?fatty?acid?esters?(3-MCPDE).?The?results?showed?that?the?acetonitrile?extract?of?oil?sample?was?separated?by?SunFireTM?C18?column?(4.6?mm×150?mm,?5?μm),?then?different?polar?components?in?oil?sample?could?be?separated?under?the?HPLC?conditions?of?mobile?phase?acetonitrile-isopropanol(volume?ratio?1∶1)，?isocratic?elution,?column?temperature?30℃?and?flow?rate?1?mL/min,?thereby?the?interfering?components?were?removed?effectively?in?the?determination?of?3-MCPDE.?The?recovery?experiments?were?conducted?by?adding?800,?1?000?mg/kg?and?1?200?mg/kg?of?3-MCPDE?standards?to?self-made?oil?sample?respectively,?the?recovery?rates?of?1,2-dioleoyl-3-chloropropanediol?and?1-stearoyl-3-chloropropanediol?were?86.4%-113.7%?and?88.4%-105.6%?respectively,and?the?relative?standard?deviations?were?less?than?15%.Therefore,?it?was?feasible?for?removing?the?interfering?components?from?oil?samples?in?determination?of?3-MCPDE?by?C18?column?and?solid?phase?extraction?method?with ?few?solvent?consumption?and?short?processing?cycle. |
| View full text View/Add Comment Download reader |
| Close |
|
|
|
