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| 免疫亲和柱净化-光化学衍生高效液相色谱荧光法测定植物油中黄曲霉毒素B1的含量 |
| Determination of aflatoxin B1 in vegetable oil by HPLC-FLD with photochemical derivatization after immunoaffinity column purification |
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| DOI： |
| 中文关键词: 高效液相色谱荧光法 光化学衍生 黄曲霉毒素B1 植物油 |
| 英文关键词:HPLC-FLD photochemical derivatization aflatoxin B1 vegetable oil |
| 基金项目: |
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| 中文摘要: |
| 建立了免疫亲和柱净化-光化学衍生高效液相色谱荧光法测定植物油中黄曲霉毒素B1含量的方法，并对样品的测定条件进行了优化。结果表明：植物油的称样量缩减为5.00?g，黄曲霉毒素B1测定结果与GB/T?18979—2003测定结果基本吻合；工作曲线在1~40?ng/mL质量浓度范围内具有良好的线性关系，相关系数为0.999?9，方法检出限为0.3?μg/kg；在空白样品中添加低、中、高3个不同添加量水平的黄曲霉毒素B1标准品，其回收率在90.5%~99.8%之间，相对标准偏差在6.3%~9.8%之间。采用光化学衍生，操作简单，无需衍生剂，避免了柱前衍生和柱后衍生受衍生剂浓度、温度、反应时间的影响，所建立的方法适用于植物油中黄曲霉毒素B1含量的测定。 |
| 英文摘要: |
| Aflatoxin?B1?in?vegetable?oil?was?determined?by?HPLC-FLD?with?photochemical?derivatization?after?immunoaffinity?column?purification,?and?the?determination?conditions?of?sample?were?optimized.The?results?showed?that?the?aflatoxin?B1?in?vegetable?oil?determined?by?the?established?method?coincided?with?the?results?determined?by?the?national?standard?method?GB/T?18979—2003?when?the?mass?of?sample?was?reduced?to?5.00?g.The?standard?curve?had?a?good?linearity?when?the?aflatoxin?B1?content?was?in?the?range?of?1-?40?ng/mL.?The?correlation?coefficient,?the?limit?of?detection,?the?recovery?rate?and?the??relative?standard?deviation?of?the?method?were?0.999?9,0.3?μg/kg,90.5%-99.8%?and?6.3%-9.8%?respectively.?The?method?had?items?of?simple?operation,?no?derivatization?agent,avoidance?of?the?influences?of?derivatization?agent?concentration,?temperature?and?reaction?time?in?the?processes?of?pre-column?and?post-column?derivatizations,?which?was?applicable?to?determine?the?content?of?aflatoxin?B1?in?vegetable?oil. |
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