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| Effect of different extraction processes on oil productivity of yeast |
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| DOI： |
| KeyWord:oil-producing yeast JM-D cell crushing oil extraction acid-hot method Soxhlet extraction |
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| Abstract: |
| The effects of different oil extraction processes on oil productivity of oil-producing yeast were studied. With high oil-producing yeast strains JM-D as raw material and oil productivity as indicator，the crushing effects of JM-D cell wall by different methods （snail enzymatic method，ultrasonic crushing method，repeated freeze-thaw method，cell homogenizer crushing method，microwave crushing method，grinding method and acid-hot method） were compared，and the extraction effects of oil from JM-D cell by organic solvent extraction method and Soxhlet extraction method were also compared. The results showed that the crushing effect of JM-D cell wall by snail enzymatic method was the best，the oil productivity reaching 46.2%，followed by repeated freeze-thaw method（thawing in 100℃water bath） and acid-hot method combined with grinding method，and their oil productivities reached 43.4% and 37.8% respectively. The extraction effect of oil from JM-D cell after grinding by Soxhlet extraction method was superior to that by organic solvent extraction method. |
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