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| 不同提取工艺对产油酵母油脂产率的影响 |
| Effect of different extraction processes on oil productivity of yeast |
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| DOI： |
| 中文关键词: 产油酵母JM-D 细胞破碎 油脂提取 酸热法 索氏抽提 |
| 英文关键词:oil-producing yeast JM-D cell crushing oil extraction acid-hot method Soxhlet extraction |
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| 中文摘要: |
| 研究了不同的油脂提取工艺对产油酵母油脂产率的影响。以高产油酵母JM-D为原料，以油脂产率为测定指标，比较了蜗牛酶法、超声波破碎法、反复冻融法、细胞匀浆机破碎法、微波破碎法、研磨法以及酸热法对酵母细胞壁的破碎效果，并对有机溶剂提取法与索氏抽提法提取细胞内油脂的效果进行比较。结果显示，蜗牛酶法的细胞破碎效果最好，油脂产率高达46.2%；其次是反复冻融（100℃水浴解冻）法，油脂产率高达43.4%;研磨法+酸热法的油脂提取效果也较好，油脂产率达到37.8%。研磨后，采用索氏抽提法的提取效果优于有机溶剂提取法的。 |
| 英文摘要: |
| The effects of different oil extraction processes on oil productivity of oil-producing yeast were studied. With high oil-producing yeast strains JM-D as raw material and oil productivity as indicator，the crushing effects of JM-D cell wall by different methods （snail enzymatic method，ultrasonic crushing method，repeated freeze-thaw method，cell homogenizer crushing method，microwave crushing method，grinding method and acid-hot method） were compared，and the extraction effects of oil from JM-D cell by organic solvent extraction method and Soxhlet extraction method were also compared. The results showed that the crushing effect of JM-D cell wall by snail enzymatic method was the best，the oil productivity reaching 46.2%，followed by repeated freeze-thaw method（thawing in 100℃water bath） and acid-hot method combined with grinding method，and their oil productivities reached 43.4% and 37.8% respectively. The extraction effect of oil from JM-D cell after grinding by Soxhlet extraction method was superior to that by organic solvent extraction method. |
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