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| Two-step solid fermentation process of rapeseed meal |
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| KeyWord:rapeseed meal detoxification two-step fermentation |
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| Abstract: |
| In order to improve the forage nutritional value of rapeseed meal，the two-step solid fermentation technology was employed to treat rapeseed meal，and the process parameters were optimized by orthogonal experiment. Three strains were screened out from eight microbial strains for aerobic fermentation. The optimal process parameters of aerobic fermentation were obtained as follows：ratio of Saccharomyces cerevisiae to Lactobacillus to Bacillus subtilis 2∶1∶1，inoculation dosage 1.5%，fermentation temperature 36℃，ratio of material to water 1∶1，fermentation time 3 d. Anaerobic fermentation of Lactobacillus was conducted based on the aerobic fermentation，and the optimal process parameters of anaerobic fermentation were obtained as follows：inoculation dosage 0.5%，fermentation temperature 33℃，fermentation time 3 d. Under these conditions，the contents of peptide，total acid and crude protein in the fermented rapeseed meal improved by 348.19%，255.28% and 7.45% respectively；the degradation rates of glucosinolates，ITC，OZT，crude fiber and phytic acid were 48.19%，26.92%，92.86%，1.35% and 14.73% respectively；tannin content increased slightly. |
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