| The accurate and efficient method for determing β-sitosterol content of oil mixture by HPLC was established,and the extraction process of β-sitosterol from oil mixture was optimized.C18 reverse-phase chromatography column was used for the determination of β-sitosterol content under the conditions of mobile phase methanol - water (volume ratio 98.5∶1.5 ) and detection wavelength 210 nm. The results showed that the mass concentration of β-sitosterol had a good linear relationship with peak area in the range of 25-500 μg/mL. The correlation coefficient, the instrumental limit of detection and the relative standard deviation (RSD) were 0.999 8, 7.5 μg/mL and 0.85% respectively. The optimal extraction conditions of β-sitosterol from oil mixture were obtained as follows: extraction times four, centrifugal speed 2 000 r/min, saponification temperature 80℃and saponification time 210 min. Under these conditions,the recovery rate and RSD of β-sitosterol were 93.44%-96.48% and 1.70%, respectively. |
