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Extraction and antioxidant activity of camellia seed protein by different methods
  
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KeyWord:camellia seed meal  camellia seed protein  antioxidant activity
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Author NameAffiliation
CAO Wei  
PAN Changliang  
TANG Wei，etc  
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Abstract:
      The extractions of camellia seed proteins from camellia seed meal by alkaline method,salt method and buffer method were studied, and their antioxidant activities were determined. The results showed that both the yield and protein content of camellia seed protein extracted by salt method were superior to other two methods. The optimal extraction conditions of salt method were obtained as follows: ammonium sulphate concentration 0.005 mol/L, material-liquid ratio 1∶50, time 180 min and pH 10. Under these conditions, the yield of camellia seed protein was 5.24% and the protein content was 82.65%. The camellia seed proteins extracted by three methods all had antioxidant abilities on DPPH radical, hydroxyl radical and Fe3+. The scavenging rate on DPPH radical was 74.78% when the concentration of camellia seed protein extracted by salt method was 8.0 mg/mL, which was better than those extracted by alkaline method and buffer method.
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