The production conditions of peanut protein concentrate from defatte peanut protein powder by alcohol extraction were optimized by single factor experiment and orthogonal experiment and verified by scale-up experiment. The results showed that the optimal production conditions were obtained as follows: volume fraction of alcohol 65%, ratio of material to liquid 1∶8, extraction temperature 60℃, extraction time 40 min, extraction times three. Under the optimal conditions, the protein content and nitrogen solubility index (NSI) of the peanut protein concentrate were 72% and 42% respectively. Furthermore, an enlarged alcohol leached process was performed at a dose of 100 g under the optimal conditions, and the protein content and NSI of the peanut protein concentrate were 70% and 40% respectively. |