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Migration regulation of macromolecular group and lipid of Schizochytrium sp. under different oxygen supply conditions |
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KeyWord:Schizochytrium sp. triacylglycerol macromolecular group oxygen supply FTIR |
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Abstract: |
In order to study the migration regulation of intracellular lipid,carbohydrate,protein,polysaccharides and oligosaccharides of Schizochytrium sp. under different oxygen supply conditions,the migration regulation of these matters during fermentation was studied by traditional method combined with the fourier transform infrared spectroscopy (FTIR). The results showed that the method of FTIR was a reliable and rapid detection method for quantitative measurement;the relative contents of triacylglycerol and oligosaccharides during oil accumulation increased with time prolonging;the relative content of carbohydrate showed a tendency of first increase then decrease;the relative contents of polysaccharides under low oxygen supply condition increased with time prolonging,while it was opposite under high oxygen supply condition. In addition,cells could accumulate lipid with neutral lipid 95.74% under high oxygen supply condition;while cells tended to accumulate polar lipid,protein and oligosaccharides under low oxygen supply condition so as to cope with the adverse environment. |
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