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| 响应面法优化牡丹籽油的水酶法提取工艺 |
| Optimization of aqueous enzymatic extraction of peony seed oil by response surface methodology |
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| DOI： |
| 中文关键词: 牡丹籽油 响应面分析 水酶法 气相色谱-质谱 |
| 英文关键词:peony seed oil response surface analysis aqueous enzymatic method GC-MS |
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| 中文摘要: |
| 采用水酶法提取牡丹籽油，在单因素实验的基础上，应用响应面法中的Box-Behnken设计对牡丹籽油的水酶法提取工艺进行优化，并通过气相色谱-质谱联用仪对牡丹籽油的脂肪酸组成进行分析。结果表明：料液比1∶〖KG-2mm〗5.4、酶解温度52〖KG-*8〗℃、酶解pH 10.3、加酶量550 U/g为较优工艺参数，该条件下牡丹籽出油率为23.25%。牡丹籽油主要含亚麻酸、亚油酸、棕榈酸、硬脂酸4种脂肪酸，其相对含量分别为58.16%、24.05%、12.33%和3.56%。 |
| 英文摘要: |
| Peony seed oil was extracted by aqueous enzymatic method. On the basis of single factor experiment，the aqueous enzymatic extraction process of peony seed oil was optimized by the Box-Behnken design of response surface methodology，and the fatty acid composition of peony seed oil was analyzed by GC-MS. The results showed that the optimal extraction conditions were obtained as follows： ratio of solid to liquid 1∶5.4，enzymatic hydrolysis temperature 52℃，enzymatic hydrolysis pH 10.3，dosage of enzyme 550 U/g. Under these conditions，the yield of peony seed oil reached 23.25%. Linolenic acid，linoleic acid，palmitic acid and stearic acid were the main four kinds of fatty acids in peony seed oil，and their contents were 58.16%，24.05%，12.33% and 3.56%，respectively. |
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