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| Optimization of aqueous enzymatic extraction of peony seed oil by response surface methodology |
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| DOI： |
| KeyWord:peony seed oil response surface analysis aqueous enzymatic method GC-MS |
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| Abstract: |
| Peony seed oil was extracted by aqueous enzymatic method. On the basis of single factor experiment，the aqueous enzymatic extraction process of peony seed oil was optimized by the Box-Behnken design of response surface methodology，and the fatty acid composition of peony seed oil was analyzed by GC-MS. The results showed that the optimal extraction conditions were obtained as follows： ratio of solid to liquid 1∶5.4，enzymatic hydrolysis temperature 52℃，enzymatic hydrolysis pH 10.3，dosage of enzyme 550 U/g. Under these conditions，the yield of peony seed oil reached 23.25%. Linolenic acid，linoleic acid，palmitic acid and stearic acid were the main four kinds of fatty acids in peony seed oil，and their contents were 58.16%，24.05%，12.33% and 3.56%，respectively. |
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