Optimization of ultrasound-assisted enzymatic extraction of apple seed oil and its fatty acid composition
  
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KeyWord:apple seed oil  ultrasound-assisted enzymolysis  extraction  response surface methodology  GC-MS  fatty acid composition
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Author NameAffiliation
BI Yanhong  
WANG Zhaoyu  
CHEN Xiaoming,etc  
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Abstract:
      The ultrasound-assisted enzymatic extraction of apple seed oil was optimized by single factor experiment and response surface methodology, and the fatty acid composition of apple seed oil was analyzed by GC-MS. The results showed that the optimal extraction conditions were obtained as follows: compound protease dosage 1.5%( based on the substrate mass), ultrasonic frequency 28 kHz, ultrasonic time 20 min, ratio of material to liquid 1∶8.43, pH 7.43 and enzymolysis time 6.38 h.Under these conditions, the theoretical yield of apple seed oil was 31.03%. The determination result of GC-MS indicated that apple seed oil was rich in unsaturated fatty acids, and its total content reached 89.371%, in which the contents of oleic acid and linoleic acid were 36.276% and 53.095% respectively.
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