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| 膜技术分离纯化花生蛋白酶解液制备活性短肽 |
| Preparation of bioactive peptides from enzymatic hydrolysate of peanut protein via membrane separation and purification |
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| DOI： |
| 中文关键词: 膜分离 超滤 纳滤 酶解液 肽 生物活性 血管紧张素转化酶 抗氧化活性 |
| 英文关键词:membrane separation ultrafiltration nanofiltration enzymatic hydrolysate peptide bioactivity angiotensin-converting enzyme antioxidant activity |
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| 中文摘要: |
| 对膜技术在花生蛋白酶解液分离纯化应用的工艺进行了研究。考察了超滤膜截留相对分子质量（1、3、5 kDa）、超滤压力（0.086、0.121、0.157、0.193 MPa）、超滤温度（30、35、40、45℃）、超滤加水量（0.5~5.5倍）对超滤效果的影响，以及考察加水次数（1~12次）对纳滤脱盐效果的影响。结果表明：超滤操作的最适工艺为用截留相对分子质量为1 kDa的超滤膜，在超滤压力0.193 MPa、超滤温度45℃的条件下进行恒体积超滤，加水量以加入3倍水为最适，此时短肽透过率可达65.01%，透过液中短肽的相对分子质量主要分布在283~402 Da之间；纳滤操作在纳滤温度为常温（20℃）、纳滤压力为1.5 MPa的条件下进行，采用间歇式恒容纳滤方式，最适加水次数为10次，此时Na+脱除率为（69.78±0.69）%，短肽损失率仅为2.00%。采用最适的先超滤、后纳滤联用工艺制备的花生短肽液经冻干后，冻干粉对ACE的IC50为0.78 mg/mL，体外抗氧化活性为ORAC值（以Trolox计）（2 359.50±40.43）μmol/g，表明短肽同时具有较强的ACE抑制活性和体外抗氧化活性。 |
| 英文摘要: |
| The application of membrane technique in separation and purification of peanut protein enzymatic hydrolysate was studied. The impacts of retention relative molecular weight of ultrafiltration membrane （1 kDa，3 kDa，5 kDa），ultrafiltration pressure （0.086 MPa，0.121 MPa，0.157 MPa，0.193 MPa），ultrafiltration temperature （30℃，35℃，40℃，45℃）and water dosage（0.5-5.5 times of peanut protein enzymatic hydrolysate） on ultrafiltration effect were discussed，and the influence of water addition times （1-12 times） on desalination effect of nanofiltration was also discussed. The results showed that the optimal ultrafiltration conditions were obtained as follows：retention relative molecular weight of ultrafiltration membrane 1 kDa，ultrafiltration pressure 0.193 MPa，ultrafiltration temperature 45℃，water dosage 3 times of peanut protein enzymatic hydrolysate with constant volume ultrafiltration. Under these conditions，the transmittance rate of short peptides was up to 65.01%，and the distribution of relative molecular weight of short peptides in the permeate was in the range of 283-402 Da. The optimal nanofiltration conditions were obtained as follows：nanofiltration temperature room temperature（20℃），nanofiltration pressure 1.5 MPa，water addition times 10 times with interval constant volume nanofiltration. Under these conditions，the removal rate of Na+was （69.78±0.69）%，while the loss rate of short peptides was only 2.00%. The short peptides prepared by the process of first ultrafiltration then nanofiltration were freeze-dried into peptides powder，the IC50 value to angiotensin-converting enzyme （ACE）and oxygen radical absorbance capacity （ORAC） value of the peptides powder were 0.78 mg/mL and （2 359.50±40.43） μmolTrolox/g respectively，which indicated that the short peptides had good ACE inhibitory activity and in vitro antioxidant activity. |
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