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| Preparation of bioactive peptides from enzymatic hydrolysate of peanut protein via membrane separation and purification |
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| DOI： |
| KeyWord:membrane separation ultrafiltration nanofiltration enzymatic hydrolysate peptide bioactivity angiotensin-converting enzyme antioxidant activity |
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| Abstract: |
| The application of membrane technique in separation and purification of peanut protein enzymatic hydrolysate was studied. The impacts of retention relative molecular weight of ultrafiltration membrane （1 kDa，3 kDa，5 kDa），ultrafiltration pressure （0.086 MPa，0.121 MPa，0.157 MPa，0.193 MPa），ultrafiltration temperature （30℃，35℃，40℃，45℃）and water dosage（0.5-5.5 times of peanut protein enzymatic hydrolysate） on ultrafiltration effect were discussed，and the influence of water addition times （1-12 times） on desalination effect of nanofiltration was also discussed. The results showed that the optimal ultrafiltration conditions were obtained as follows：retention relative molecular weight of ultrafiltration membrane 1 kDa，ultrafiltration pressure 0.193 MPa，ultrafiltration temperature 45℃，water dosage 3 times of peanut protein enzymatic hydrolysate with constant volume ultrafiltration. Under these conditions，the transmittance rate of short peptides was up to 65.01%，and the distribution of relative molecular weight of short peptides in the permeate was in the range of 283-402 Da. The optimal nanofiltration conditions were obtained as follows：nanofiltration temperature room temperature（20℃），nanofiltration pressure 1.5 MPa，water addition times 10 times with interval constant volume nanofiltration. Under these conditions，the removal rate of Na+was （69.78±0.69）%，while the loss rate of short peptides was only 2.00%. The short peptides prepared by the process of first ultrafiltration then nanofiltration were freeze-dried into peptides powder，the IC50 value to angiotensin-converting enzyme （ACE）and oxygen radical absorbance capacity （ORAC） value of the peptides powder were 0.78 mg/mL and （2 359.50±40.43） μmolTrolox/g respectively，which indicated that the short peptides had good ACE inhibitory activity and in vitro antioxidant activity. |
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