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| 松仁蛋白酶解液体外抗氧化活性分析 |
| In vitro antioxidant activity of pine kernel protein hydrolysate |
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| DOI： |
| 中文关键词: 松仁蛋白 酶解液 胃蛋白酶 胰蛋白酶 抗氧化活性 |
| 英文关键词:pine kernel protein enzymatic hydrolysate pepsin trypsin antioxidant activity |
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| 中文摘要: |
| 采用两种胃肠消化酶（胃蛋白酶、胰蛋白酶）对松仁蛋白进行酶解实验，研究其胃蛋白酶酶解液、胰蛋白酶酶解液和胃蛋白酶-胰蛋白酶分步酶解酶解液的体外抗氧化活性。结果表明：胃蛋白酶酶解30 min的酶解液具有最强体外抗氧化活性，·OH清除率为67.43%，O-2·清除率为55.66%；胰蛋白酶酶解60 min的酶解液具有最强清除·OH能力，·OH清除率为68.79%；胰蛋白酶酶解120 min的酶解液具有最强清除O-2·能力，O-2·清除率为39.20%；胃蛋白酶-胰蛋白酶分步酶解松仁蛋白酶解液在酶解240 min时，具有最强的体化抗氧化活性，此时·OH清除率为79.11%，O-2·清除率为68.99%。 |
| 英文摘要: |
| Enzymatic hydrolysis experiment of pine kernel protein was conducted by two kinds of gastrointestinal digestive enzymes （pepsin and trypsin），and in vitro antioxidant activities of enzymatic hydrolysates of pine kernel protein obtained by hydrolysis of pepsin，trypsin and pepsin-trypsin were studied. The results showed that after enzymatic hydrolysis for 30 min，the pepsin enzymatic hydrolysate of pine kernel protein had the strongest in vitro antioxidant activity，and the scavenging rates on ·OH radical and O-2·radical were 67.43% and 55.66% respectively；the trypsin enzymatic hydrolysate of pine kernel protein had the strongest scavenging capacity on ·OH radical after enzymatic hydrolysis for 60 min，and the scavenging rate on ·OH radical was 68.79%，while it had the strongest scavenging capacity on O-2· radical after enzymatic hydrolysis for 120 min，and the scavenging rate on O-2· radical was 39.20%；pepsin-trypsin step-by-step enzymatic hydrolysate of pine kernel protein had the strongest in vitro antioxidant activity after enzymatic hydrolysis for 240 min，and the scavenging rates on·OH radical and O-2·radical were 79.11% and 68.99% respectively. |
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