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Immobilization of Lipozyme TL 100L and its application in synthesis of diglycerides by glycerolysis |
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KeyWord:immobilization lipase diglycerides glycerolysis |
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Abstract: |
Lipozyme TL 100L was immobilized by four kinds of common macroporous resins(NKA-9,AB-8,D301R,D4020). The immobilization effects of the four kinds of common macroporous resins were compared,and the impacts of four kinds of the immobilized lipases on synthesis of diglycerides by glycerolysis were also compared. The results showed that immobilized Lipozyme TL 100L with macroporous resin AB-8 as carrier had the highest catalytic efficiency in synthesis of diglycerides by glycerolysis of soybean oil in a solvent free system. When dosage of immobilized Lipozyme TL 100L with macroporous resin AB-8 as carrier was 600 U/g,molar ratio of refined soybean oil to glycerol was 1∶2 and reaction temperature was 50℃,the content of diglycerides in the product reached 54.39% after glycerolysis for 6 h,in which the contents of 1,2-diacylglycerol and 1,3-diacylglycerol were 33.87% and 20.52% respectively. |
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