The olive polyphenols (OPP) were separated from olive oil processing effluent using AB-8 macroporous resin. The process conditions of static adsorption, static desorption and dynamic elution were investigated respectively. The results showed that the optimal static adsorption conditions were obtained as follows: dosage of pretreated resin 1.5 g, adsorption time 3 h and adsorption temperature 30℃, under these conditions,the adsorption capacity of 20 mL crude extract diluent (OPP mass concentration 1.52 mg/mL) reached 14.43 mg/g; the optimal dynamic elution conditions were obtained as follows: elution rate 1.0 mL/min, subsection elution with water, 70% and 90% of ethanol solutions. Under the combined condition of static adsorption and dynamic subsection elution, the purity of OPP after purification was 56.44%,which was 7.93 times as high as that of crude extract diluent. |