| The effect of enzyme combined with ultrasound treatments on the extraction of polysaccharides from rice bran was investigated. The extraction conditions of polysaccharides from rice bran were optimized by response surface methodology,and antioxidant activity of rice bran polysaccharides was researched. The results showed that both ultrasound and compound enzymes (mass ratio of cellulase to neutral protease 1∶1) treatments could improve the extraction of rice bran polysaccharides. The optimal extraction conditions of rice bran polysaccharides were obtained as follows: dosage of compound enzymes 3.1 mg/mL, enzymolysis time 2 h, ultrasonic power 198 W, ultrasonic time 20 min, ratio of material to liquid 1∶30, extraction time 3.2 h, extraction temperature 60 ℃. Under these conditions, the yield of rice bran polysaccharides reached 5.3%. The rice bran polysaccharides had strong reducing power and certain antioxidant capability on oil and its scavenging effects on DPPH· and ·OH were significant. |
