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Screening of high lipid production strain from Rhodotorula glutinis mutagenized by atmospheric and room temperature plasma and optimization of fermentation condition
  
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KeyWord:atmospheric and room temperature plasma  Rhodotorula glutinis  mutation  biodiesel  fermentation
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Author NameAffiliation
LIU Yating  
LIU Hongjuan  
WANG Yanping  
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Abstract:
      The Rhodotorula glutinis was mutagenized by atmospheric and room temperature plasma (ARTP). The optimal mutation condition was 60 s at 120 W,under this condition the lethality rate was 90%. The strain LA1 with high lipid production was screened from mutagenized Rhodotorula glutinis by Sudan black B staining method. The lipid productivity and lipid content of LA1 increased by 17.59% and 46.08% respectively comparing with Rhodotorula glutinis.The fermentation conditions of LA1 were optimized as follows: initial mass concentration of glucose 70 g/L, with (NH4)2SO4 as nitrogen source and C/N 53. Under these conditions, the lipid productivity, biomass, lipid content and lipid yield of LA1 increased by 123.9%,56.6%,43.0% and 43.5% respectively, and the fatty acid composition of the lipid of LA1 was similar to that of vegetable oil.
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