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| 常压室温等离子体诱变粘红酵母筛选高产油脂菌株及发酵条件优化 |
| Screening of high lipid production strain from Rhodotorula glutinis mutagenized by atmospheric and room temperature plasma and optimization of fermentation condition |
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| DOI： |
| 中文关键词: 常压室温等离子体 粘红酵母 诱变 生物柴油 发酵 |
| 英文关键词:atmospheric and room temperature plasma Rhodotorula glutinis mutation biodiesel fermentation |
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| 中文摘要: |
| 以粘红酵母（Rhodotorula glutinis）为出发菌株，采用常压室温等离子体对其进行诱变。得到最佳诱变条件为120 W功率下处理60 s，致死率达90%。诱变后，采用苏丹黑B染色法筛选出1株高产油脂突变株LA1，与出发菌株相比较，其油脂产量、油脂含量分别较出发菌株提高了17.59%和46.08%。对LA1发酵培养条件进行优化，得到最佳条件为初始葡萄糖质量浓度70 g/L，氮源为（NH4）2SO4，C/N值53。在最佳发酵条件下，LA1的油脂产量、生物量、油脂含量及油脂得率较优化前分别提高了123.9%、56.6%、43.0%和43.5%，其油脂脂肪酸组成与植物油相近。 |
| 英文摘要: |
| The Rhodotorula glutinis was mutagenized by atmospheric and room temperature plasma (ARTP). The optimal mutation condition was 60 s at 120 W,under this condition the lethality rate was 90%. The strain LA1 with high lipid production was screened from mutagenized Rhodotorula glutinis by Sudan black B staining method. The lipid productivity and lipid content of LA1 increased by 17.59% and 46.08% respectively comparing with Rhodotorula glutinis.The fermentation conditions of LA1 were optimized as follows: initial mass concentration of glucose 70 g/L, with (NH4)2SO4 as nitrogen source and C/N 53. Under these conditions, the lipid productivity, biomass, lipid content and lipid yield of LA1 increased by 123.9%,56.6%,43.0% and 43.5% respectively, and the fatty acid composition of the lipid of LA1 was similar to that of vegetable oil. |
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