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| Determination of free gossypol in cottonseed meal by HPLC |
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| KeyWord:cottonseed meal HPLC free gossypol |
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| Abstract: |
| The determination method of free gossypol （FG） in cottonseed meal was established by high performance liquid chromatography （HPLC）. The results showed that the optimal HPLC conditions were obtained as follows：mobile phase V（methanol +0.2% H3PO4）∶V（V（acetonitrile）∶V（water ）=1∶3）=85∶15 （respective using two injectors），column pressure 16.2 MPa，column temperature 25℃，flow rate 1.0 mL/min，injection volume 20 μL，Waters XTerra MS C18 stainless steel chromatographic column (250 mm×4.6 mm，5 μm)，UV detection wavelength 235.5 nm. The FG in cottonseed meal could be well detected under the optimal conditions. A good linear relationship between peak area and mass concentration of standard gossypol solution was observed in the range of 0.15-5 μg/mL（R2=0.999 9）. The mean recovery was 95.67%-98.99% with relative standard deviation ranging from 1.28% to 2.03%. |
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