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| 水相体系酶法制备溶血磷脂酰乙醇胺研究 |
| Enzymatic preparation of lysophosphatidylethanolamine in aqueous media |
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| DOI： |
| 中文关键词: 磷脂酰乙醇胺 溶血磷脂酰乙醇胺 磷脂酶A1 酶解反应 |
| 英文关键词:phosphatidylethanolamine lysophosphatidylethanolamine phospholipase A1 enzymolysis reaction |
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| 中文摘要: |
| 探索了磷脂酶A1在水相体系中酶解制备溶血磷脂酰乙醇胺（LPE）的可行性，并进行了酶解条件对LPE相对含量的影响研究。得到最佳酶解条件为：磷脂酰乙醇胺（PE）的纯度为80%，液料比10∶1，酶解温度35℃，酶添加量0.04 mL/g，CaCl2添加量0.010 g/mL，酶解时间30 min。在最佳酶解条件下，得到酶解产物中的LPE相对含量为42.0%。 |
| 英文摘要: |
| The feasibility of enzymatic preparation of lysophosphatidylethanolamine (LPE) by phospholipase A1 in aqueous media was studied, and the effects of enzymolysis conditions on the relative content of LPE were discussed. The results showed that the optimal enzymolysis conditions were obtained as follows: purity of phosphatidylethanolamine (PE) 80%, ratio of liquid to material 10∶1, enzymolysis temperature 35 ℃, dosage of phospholipase A1 0.04 mL/g, dosage of CaCl2 0.010 g/mL,enzymolysis time 30 min.Under the optimal conditions, the relative content of LPE in the enzymatic hydrolysate reached 42.0%. |
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