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Determination of lipid content in Botryococcus braunii by Nile red-fluorescence spectrometry
  
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KeyWord:Botryococcus braunii  lipid  Nile red  fluorescence spectrometry method
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Author NameAffiliation
BING Xin  
SONG Qi  
LIU Shuang，ect  
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Abstract:
      The traditional Nile red fluorescence dyeing method could not be applied to determine the lipid content in Botryococcus braunii because of its extracellular excretion, thick cell wall and dense cell colonies. By studying the pretreatment and staining conditions, the detection method was optimized as follows: treating algal cells by ultrasound for 3 min at 20℃,100 W and 40 kHz, centrifugation at 3 000×g to remove the culture medium, suspending the cells with 8% dimethylsulfoxide to OD7501.6, every 1 mL sample added by 10 μL Nile red acetone solution of 120 μg/mL (mass concentration of Nile red 1.2 μg/mL), staining at 40℃ for 10 min. Then the sample was measured by fluorescence spectrometer excited at 520 nm. The interaction between Nile red and microalgal cells, and the determination stability and accuracy were enhanced after optimization.
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