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| Enzymatic extraction of squid viscera oil and its fatty acid analysis |
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| KeyWord:squid viscera fish oil animal protease response surface methodology fatty acid composition |
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| Abstract: |
| The extraction process of Argentina squid viscera oil by animal protease was researched. With the extraction rate of squid viscera oil as index，the extraction conditions were optimized by response surface methodology on the basis of single factor experiment，and the physicochemical properties and fatty acid composition of squid viscera oil were analyzed. The results showed that the optimal extraction conditions of squid viscera oil were obtained as follows：dosage of enzyme 2.0%，pH 7.5，enzymolysis temperature 50℃，mass ratio of solid to liquid 1∶1.0，enzymolysis time 4 h. Under the optimal extraction conditions，the extraction rate of squid viscera oil reached 78.83%. The color of squid viscera oil was reddish brown, it had the fishy smell without rancid odor，and its physicochemical properties reached the first grade standard of crude fish oil (SC/T 3502—2000). The content of polyunsaturated fatty acids was up to 41.66%，in which the total content of EPA and DHA was 35.42%. |
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